2009 Study Abstract
Perinatal exposure to diethylstilbestrol (DES) can have numerous adverse effects on the reproductive organs later in life, such as vaginal clear-cell adenocarcinoma. Epigenetic processes including DNA methylation may be involved in the mechanisms.
We subcutaneously injected DES to neonatal C57BL/6 mice. At days 5, 14, and 30, expressions of DNA methyltransferases (Dnmts) Dnmt1, Dnmt3a, and Dnmt3b, and transcription factors Sp1 and Sp3 were examined. We also performed restriction landmark genomic scanning (RLGS) to detect aberrant DNA methylation. Real-time RT-PCR revealed that expressions of Dnmt1, Dnmt3b, and Sp3 were decreased at day 5 in DES-treated mice, and that those of Dnmt1, Dnmt3a, and Sp1 were also decreased at day 14. RLGS analysis revealed that 5 genomic loci were demethylated, and 5 other loci were methylated by DES treatment. Two loci were cloned, and differential DNA methylation was quantified.
Our results indicated that DES altered the expression levels of Dnmts and DNA methylation.
- Neonatal exposure to diethylstilbestrol alters expression of DNA methyltransferases and methylation of genomic DNA in the mouse uterus, Sato K1, Fukata H, Kogo Y, Ohgane J, Shiota K, Mori C. Endocr J. 2009;56(1):131-9. Epub 2008 Nov 8. NCBI PMID: 18997445.
- Full text, The Japan Endocrine Society, PDF.
More DES DiEthylStilbestrol Resources
- DES studies on cancers and screening.
- DES studies on epigenetics and transgenerational effects.
- DES studies on fertility and pregnancy.
- DES studies on gender identity and psychological health.
- DES studies on in-utero exposure to DES and side-effects.
- DES studies on the genital tract.
- Papers on DES lawsuits.
- DES videos and posts tagged DES, the DES-exposed, DES victims.
15 thoughts on “Neonatal exposure to DES alters expression of DNA methyltransferases and methylation of genomic DNA”
Thanks For Sharing Dom
you are welcome Gerrit
Reblogged this on Barrow Blogs.
many thanks Judith
Reblogged this on Smorgasbord – Variety is the spice of life and commented:
In a follow up to Judith Barrow’s Sunday Show when she talked about Diethylstilbestrol and its adverse effects here is an extremely important post by Desdaughter
many thanks Sally
My pleasure very important message and information.
If you have a summary of the impact of this research, I’d appreciate getting that. This does not offer any actual information as to what it all signifies.
Hi Sally. I think this study aims to show some of the why and the how => about DES being transmittable through several generations…
Oh. Thanks. I already knew the why and how. I wanted to know more about what the impact of all that is on generations of girls (or boys?).
Sally, I am not aware of a study which details all side effects impacts on several generations…
But for example, a doctor said that while hyspospadias was affecting +/- 3.5 % of the DES sons, the risk was increasing to reach 8% on the DES grand sons… so, that risk doubles with the next generation… here is the video, listen from 08:30 to 09:05.
While here is a chart for daughters, I’d like to find one comparing 3 generations…
So far, studies I found are listed here.
Hope this helps
Thanks so much!
Reblogged this on Michaelphelps1's Blog and commented:
FACINATING! If not for Sorgasboard . . . many, many people who need to know this would have missed it!
many thanks Michael